| 1 | Alewife | Alosa pseudoharengus |
| 2 | American eel | Anguilla rostrata |
| 3 | American shad | Alosa sapidissima |
| 4 | Atlantic salmon | Salmo salar |
| 5 | Aurora trout | Salvelinus fontinalis timagamiensis |
| 6 | Bowfin | Amia calva |
| 7 | Brook trout | Salvelinus fontinalis |
| 8 | Brown trout | Salmo trutta |
| 9 | Burbot | Lota lota |
| 10 | Catfish | Family: Ictaluridae |
| 11 | Channel catfish | Ictalurus punctatus |
| 11.1 | Cisco (Lake herring) | Coregonus artedi |
| 12 | Ciscoes (Chubs), except Lake herring | Coregonus hoyi, C. reighardi, C. kiyi, C. nigripinnis and C. zenithicus |
| 13 | Common carp | Cyprinus carpio |
| 14 | Crappie (black crappie and white crappie) | Pomoxis nigromaculatus and Pomoxis annularis |
| 15 | Crayfish | Orconectes spp., Fallicambarus spp. and Cambarus spp. |
| 16 | Darter | Any species of the genera: Ammocrypta, Etheostoma, and Percina native to Ontario |
| 17 | Freshwater drum | Aplodinotus grunniens |
| 18 | Gar | Family: Lepisosteidae |
| 19 | Gizzard shad | Dorosoma cepedianum |
| 20 | Goldeye | Hiodon alosoides |
| 21 | Goldfish | Carassius auratus |
| 22 | Grass pickerel | Esox americanus vermiculatus |
| 23 | Kokanee | Oncorhynchus nerka |
| 24 | [Repealed, SOR/2014-311, s. 13] |
| 25 | Lake sturgeon | Acipenser fulvescens |
| 26 | Lake trout | Salvelinus namaycush |
| 27 | Lake whitefish | Coregonus clupeaformis |
| 28 | Largemouth bass | Micropterus salmoides |
| 29 | Minnow | Any species of family: Cyprinidae that is native to Ontario except common carp or goldfish |
| 30 | Mooneye | Hiodon tergisus |
| 31 | Mudminnow | Family: Esocidae |
| 32 | Muskellunge | Esox masquinongy and the hybrid of the muskellunge and the northern pike, E. masquinongy x E. lucius |
| 33 | Northern pike | Esox lucius |
| 34 | Pacific salmon (a) Chinook salmon (b) Coho salmon (c) Pink salmon
| Any species of the genus: Oncorhynchus, except rainbow trout |
| 35 | Panfish | Family: Centrarchidae except Micropterus salmoides, M. dolomieu, Pomoxis annularis and P. nigromaculatus |
| 36 | Rainbow smelt | Osmerus mordax |
| 37 | Rainbow trout | Oncorhynchus mykiss |
| 38 | Rock bass | Ambloplites rupestris |
| 39 | Round whitefish | Prosopium cylindraceum |
| 40 | Sauger | Sander canadensis |
| 41 | Sculpin | Family: Cottidae |
| 42 | Smallmouth bass | Micropterus dolomieu |
| 43 | Splake (backcross) | The hybrid of the lake trout and the brook trout, S. namaycush x S. fontinalis |
| 44 | Stickleback | Family: Gasterosteidae |
| 45 | Sucker | Family: Catostomidae |
| 46 | Sunfish | Any species of the genus: Lepomis |
| 47 | Trout-perch | Percopsis omiscomaycus |
| 48 | Walleye (yellow pickerel) | Sander vitreus and the hybrid of walleye and sauger, S. vitreus x S. canadensis |
| 49 | White bass | Morone chrysops |
| 50 | White perch | Morone americana |
| 51 | Yellow perch | Perca flavescens |